Re: Molecular Probes Cytotox-Kit Question

Andreas Schrer (
Thu, 3 Oct 96 22:57:52 +0100

>It depends on what you are trying to interpret! The only aggregate that =
>confuse a calculation of % of targets killed is an aggregate between a =
>target cell and a dead effector. This seems an unlikely occurance under =
>circumstances, and you can get an indirect feel for this by finding the =
>of PI-positive DiO-negative events (it should be quite low).

Well, you are absolutely right with this, but the authors of the =
cited paper
themselves wrote about this confusion, so I'm still a bit skeptical. =
if I gated out aggregates and right in these aggregates were a higher =
of vital targets (i.e. not yet killed targets but targets adherent to =
in comparison to the singlet cells (i.e. more already killed targets =
not anymore
adherent to effectors), well okay, I'm probably a bit too skeptical.

>Finally, if you really think you will have dead effectors you could mark =
>population also before the co-incubation or by post-staining with a =
>specific mAb.

Yes, by using a marker mAb you should be able to clarify the =

Thank you very much for your reply.

Andreas Schr=F6der

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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web , EMAIL