RE: THP-1's and fluorescent dyes

Dan Smith (DJS@ALLP.COM)
Tue, 17 Dec 96 09:25:00 PST

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I have tried to use THP-1's in a cytochrome-C oxidative burst experiment and
had absolutely no luck. They may produce little to no superoxide.
Dan Smith
San Diego
----------
{From: owner-cyto-sendout
{To: Cytometry Mailing List
{Subject: THP-1's and fluorescent dyes
{Date: Monday, December 16, 1996 6:08PM
{
{
{
{ Has anyone worked with the THP-1 cell line using Dihydrorhodamine 123
{(DHR)
{ or Hydroethidine (HE)? I am in search of a method to stimulate these
{cells
{ with LPS, an inflammatory cytokine, or virus, and measure oxidative
{burst.
{ As of yet, my efforts have come up empty, but I thought there may be
{some
{ unpublished or hard to find methods out there in Flowland. Any help,

{
{ insights or watch-outs will be appreciated.
{
{ Thanks...Jodi McKenzie-Kroeger
{

Next message: ctnebe: "unusual T cell phenotype/absolute counts"
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu