Re: Fluorescent tags for free DNA

Tue, 24 Sep 1996 09:18:58 GMT0BST

Dear All,

Robin Barclay wrote:

> We want to tag free DNA to follow its possible delivery into cells

May I suggest that you look at the following paper:

Flow cytometric quantification of electroporation-mediated uptake of
macromolecules into plant protoplasts.
Blackhall N.W., Finch R.P., Power J.B., Cocking E.C. and Davey M.R.
Protoplasma (1995) 186:50-56

This paper looks at the uptake of FITC-Dextran and uses PI to assess
viability. In this work we were able to correlate dextran uptake
with transient gene expression following uptake using the same

Please contact me if you need further assistance

Nigel Blackhall

Plant Genetic Manipulation Group,
Department of Life Science,
University of Nottingham, University Park, Nottingham NG7 2RD

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