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I will refer you to our paper.
Marie D, Partensky F, Jacquet S, Vaulot D (1997) Enumeration and cell cycle
analysis of natural populations of marine picoplankton by flow cytometry
using the nucleic acid stain SYBR Green I. Appl Environ Microbiol 63:186-193
In fact we calibrate the flow rate of the FACScan and then record the time
of each analysis. We have been using this method for several years and it
is very very accurate (a few %) provided the flow rate calibration is done
often enough (2-3 times per day).
Daniel Vaulot
______________________________________
Daniel VAULOT
Station Biologique CNRS - INSU - UPMC
BP 74
29682 Roscoff Cx FRANCE
Ph: 33 2 98 29 23 34 Fax: 33 2 98 29 23 24
e-mail: vaulot@sb-roscoff.fr
W3: http://www.sb-roscoff.fr (PhytoPlankton Group)
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web