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Using the "vertical" sort, Aaron was able to achieve greater than 90%
success--measured as PCR-positive sequences from the wells. It's likely that
the actual success in sorting was significantly higher than this, possibly close
to 100%, and that the 10% "failures" were PCR-based failures and not sorting
failures.
The most difficult obstacle is the accurate positioning of the receptacle under
the sorted drops. Here you may leave the cap on the plate, position it so that
sorted drops end up in the right place, and then take the cap off for the sort.
By the way, the vertical sorting is also useful for doing "high volume" sorts,
since it builds up the least amount of charge in the receiving tube... you have
much less "fly-outs" of sorted events.
mr
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web