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We are about to publish a paper; "Al-Rubeai, M et al. A rapid method
for evaluation of cell number and viability by flow cytometry. Cytotechnology (in
press)".
The method is based on the ratio of cells to a known number of
particles method but includes PI as a vital stain.
It has been compared with traditional trypan blue stained
haemocytometer counting and validated for use in animal cell biotechnology where
cultures may be highly heterogeneous, containing not only "normal
cells" but substantial numbers of apoptotic and necrotic cells and a
large amount of debris.
The method has advantages in speed, objectivity and low interobserver
variation and offers the potential for near online automation in
process biotechnology.
Please feel free to contact me for further information.
Regards
DR Lloyd
DR Lloyd MMedSc CBiol MIBiol
Research Associate
School of Chemical Engineering
University of Birmingham
Edgbaston
Birmingham B15 2TT
England
t; 0121 414 5264
e; D.R.Lloyd@bham.ac.uk
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web