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Unless I'm in error, open vials can be used for 9 "warming cycles" (the
refrigerated substrate need to be warmed to room temperature before staining).
Therefore, an open vial can be used nine times up through it's expiration
date, which may lengthen the period of effective use.
There are also products available from Bioergonomics (Fluorotrol) and Phoenix
Flow Systems (CRISP). These are stabilized tissue culture cell products, which
have longer "outdates".
One caveat about all three; the fluorescence intensity of the CD34-bearing
cells MAY be brighter than you are used to seeing with stem cells products,
depending on the substrates you typically analyze.
Marc Langweiler
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web