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Thanks to all that replied to my query!
I was going to post a summary, BUT - the checklist has changed.
Many thanks to Karen Munn at Duke for bringing this to my attention!!
And - Thanks to CAP for improving their checklist.
1997.0 Edition under Blood Lymphocyte Subset Enumeration states:
"Is there a procedure to set markers (cursors) to distinguish
fluorescence-negative and fluorescence-positive cell populations?"
1997.0 Edition under Leukemia and Lymphoma states:
"Is there a procedure to distinguish fluorescence negative and
fluorescence-positive cell populations?"
"NOTE: This does not imply that a separate negative control sample must
be run. It is possible to coordinate panels of monoclonal antibodies to
compare the binding of monoclonal antibodies of the same subclass that
typically have mutually exclusive patterns of reactivity of subsets of
hematopoietic cells. In this way, test antibodies may also double as
control reagents."
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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