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Using a non-tandem dye such as BD'd PerCP can get round that problem, but
it is well-documented that PerCP is photobleached at high laser powers
that are used on sorters.
The reason I am writing all this is that today I have received some
literature about a dye called ALPHA-RED which comes from Exalpha
Laboratories in the States (Through SeroTec here in the UK). I have been
told that this is not a tandem dye and that it is excited at 488nm and
emits at 670nm so it is obviously a possible alternative to PerCP.
Has anybody out there heard of this dye or used it? Any comments?
Derek
Derek Davies
FACS Laboratory,
Imperial Cancer Research Fund, London, UK
http://www.icnet.uk/axp/facs/davies/index.html
Simon wrote:
>>I too would like to be illuminated as to which dyes can be used for four
>>colour work with the Calibur, are we really stuck with PerCP as the only
>>third colour?
>>Simon
>>Simon Monard
>>FACS Lab Manager
>>Aaron Diamond Center for AIDS Research
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web