PI Staining

Gary Durack (durack@ux1.cso.uiuc.edu)
Wed, 9 Jul 1997 15:16:37 -0500 (CDT)

It is usually not necessary to wash out PI because bound PI has a much
higher quantum efficiencey than free dye. Also, most flow cytometer
electronics are designed to ignore constant, or DC background such as a
fixed concentration of free dye in solution.

gary


Gary Durack
University of Illinois Biotechnology Center
voice (217) 244-0559 fax (217) 244-0466
durack@uiuc.edu
http://www.life.uiuc.edu/biotech


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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu