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Bob,
We routinely use PI staining for live/dead discrimination. It does not need
to be washed out prior to running. In fact, PI will leach out if washed. We
add it to a tube, wait a few seconds, and then run the sample on our
FACSCAN. If you are doing PI for DNA where the cells are permeabilized, I
would imagine the same routine applies as PI is reversible in its binding
to DNA. I am sending a copy of this to Paul in hopes he will have it posted
for the group's info.
Good Luck,
Randy Fischer
NIH/NIA
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posted by
J.Paul Robinson, Purdue University Cytometry Labs
Professor of Immunopharmacology
robinson@flowcyt.cyto.purdue.edu PH:765-494 6449 FAX:765-494 0517
web http://www.cyto.purdue.edu
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web