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The guidelines stating that refrigeration would cause loss of selected
populations were created in the era of density-gradient centrifugation.
Whole blood lysis techniques avoid these losses, and therefore
refrigeration is a viable solution for specimen storage/preservation. And
if you can validate it, you can do it (i.e. store specimens you have run at
4 C then run again to validate no changes occur).
An article published in 1993 covered a wide range of storage conditions and
their effect on immunophenotyping:
Paxton H, Bendele T. Effect of time, temperature, and anticoagulant on flow
cytometric hematological values. Ann NY Acad Sci 1993;677:440-3.
good luck.
-- Keith Bahjat Northwestern University Medical School Comprehensive AIDS Center Flow Cytometry Laboratory Chicago, IL Kbahjat@nwu.edu
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web