 |
 |
 |
 |
 |
We found that fluorescence after CM-FDG staining for B-gal activity
was quite well retained after 3.7% formaldehyde fixation. We presume
that the chloromethyl moiety of CM-FDG reacts with protein thiols. It
should be noted that some of the fluorescent products of CM-FDG, and
also FDG, get lost from cells. With verapamil one can prevent that.
We have submitted a paper describing all this.
Hope this helps,
Martin Poot
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web