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> A. Fix & Perm is not the only way, but it is a good and easy way (it is
> often recommended in consensus protocols)! You can buy it from caltag
> in U.S. Most of our customers use this reagent! But it wont work for TdT
> staining!!
>
This is definitely not true. We have been using Fix & Perm
successfully for TdT staining using the Supertech mix of monoclonals
for the last five years. Any fixation procedure will induce
epitope specific alterations of the target antigens. Therefore,
any fixation and permeabilization procedure can only be
tested for specific clones. Also a consensus should always
only be achieved on methods and quality control but never
on company specific reagents. Similar reagents are by now
available from most diagnostic companies.
Gregor Rothe
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Institute for Clinical Chemistry and Laboratory Medicine
University of Regensburg
D-93042 Regensburg, Germany
Tel. +49 (941) 944-6204
Fax +49 (941) 944-6202
Internet: Gregor.Rothe@klinik.uni-regensburg.de
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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