Re: pulse processing in cell cycle analysis

Larry Seamer (LSEAMER@COBRA.UNM.EDU)
Wed, 7 May 1997 16:43:23 +0000

In response to Fang-yao Hou, peak height measurements can under measure
total fluorescence when the particle being measured has a cross-sectional
diameter larger than the effective illumination spot of the laser. in
cell-cycle analysis this situation will result in a mean channel G2/G1 ratio
less than the theoretical 2.0 and often as low as 1.8. Therefore, pulse area
measurements are preferred when doing DNA staining.

On Tue, 6 May 1997, Fang-Yao Hou wrote:

>
> Dear FLOW experts:
>
> This is to ask your opinions about using FL-height, instead of FL-area in
> cell cycle analysis. I can see typical picture of G1, S and G2/M from FL-
> height histogram as long as I acquire data in linear mode. I am wondering
> if this is an acceptable way to do cell cycle analysis without purchasing
> the pulse processing module. The cytometer I am using is the BD Vantage.
> I appreciate your responses.
>
> Fang-Yao Hou
> FYHOU@macc.wisc.edu
>


Home Page Table of Contents Sponsors E-Mail Archive Web Sites

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu