Re: Yeast Cell Cycle Method
Larry Arnold (lwarma@med.unc.edu)
Tue, 8 Apr 1997 10:50:48 -0400 (EDT)
At 01:21 PM 4/7/97 CST, you wrote:
>
>Does anyone have a good yeast cell cycle method? In particular, we need to
>eliminate a problem with the yeast clumping and try to get good single cell
>suspension. Have been using PI, but will try any method/dye that works well.
>Thank you, Martha
>Martha Kirby
>NIH, NHLBI, HB
>Bldg. 10, Rm 7C212
>Bethesda, MD 20892-1652
>kirbym@helix.nih.gov
>301-402-0277
>
>
Check out: Methods in Cell Biology : Flow Cytometry ,Second Edition Part B
, Chapter 27p 458. Several of our investigators have used this with success.
Larry Arnold
Director, Flow Cytometry Facility
Univ. of North Carolina at Chapel Hill
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
