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Thermal Denaturation of DNA for Immunochemical Staining of Incorporated
BrdUrd: Critical Factors That Affect the Amount of Fluorescence and the
Shape of BrdUrd/DNA Histogram: Cytometry 10:718-725 (1989).
On -1 xxx -1 SMTP%h82@aixterm1.urz.uni-heidelberg.de@COBRA.UNM.EDU wrote:
> Is anybody of you aware of a good (means working) protocol for
> a Br-desoxyuridine-FACS-procedure? I have used the B-D-protocol
> provided in their monoclonal antibody resource book, but without
> success :-(
> In particular I am looking for information about the FACS-setup
> for aquisition and analysis of cell proliferation and hints
> about possible pitfalls in the procedure.
>
> Any help is greatly appreciated.
>
>
> --
> Andreas Schr=F6der
>
>
>
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web