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I have recently processed several hundred histograms with 'cylchred'.
Some of these have been BrdU +ve/-ve fractions following pulse
labelling. Many of these fit the description above and others have
been more extreme. The program will attempt to find G0/1 G2/M
positions, if you are not happy with these positions values can be
called back from the previous histogram or inserted manually.
Calculation of phases is then based on Watson et al Cytometry 8 1-8
which handles synchronised populations as well as more conventional
distributions.
Version 1.0.5 uses wildcards for easy selection of groups of files
and produces a textfile of results for transferring into
spreadsheets. It is PC based but I have seen it operating on a Mac
with SoftWindows.
Copies from
http://www.cf.ac.uk/uwcm/hg/hoy/
That takes you to my home page which starts with an
illustration from Cylchred
Follow the links to 'Software from Cardiff'
If you prefer ftp
OPEN ftp.cf.ac.uk
CD /pub/UWCM/hg
GET cylchred.exe (as binary).
Terry.
Dr. Terry Hoy
Principal Research Officer
Department of Haematology Telephone 44 1222 743458
University of Wales College of Medicine FAX 44 1222 744655
Heath Park E-mail hoy@cardiff.ac.uk
CARDIFF CF4 4XN http://www.cf.ac.uk/uwcm/hg/hoy/index.html
U.K.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web