RE: Proliferation index and apoptosis

Tom Mc Closkey (
Wed, 23 Apr 97 11:30:34 PDT

--- On Tue, 22 Apr 1997 08:54:03 -0600 Anna Travelstead
<> wrote:
The PI wants
>DNA cell cycle analysis, proliferation index and apoptosis index
>performed. Does anyone have recommendations on the best apoptosis
>kit for use on a FACS Vantage?

The easiest way to measure these 3 parameters is to stain for DNA content
which allows you to quantitate apoptosis [below diploid] and proliferation
[above diploid] simultaneously. If you wish to analyze cell death using a
different method you have many choices, including TUNEL, annexin, which are
in kit form. There are also many other methods discussed in current

Good luck,

Thomas W. Mc Closkey, Ph. D.
Director, Flow Cytometry
North Shore University Hospital
Biomedical Research Center
350 Community Drive
Manhasset, Long Island, New York 11030
ph: 516-562-4844 [office]; 516-562-1135/4641 [lab]
4/23/97 11:37:08 AM

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