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Using a triple labelling method that also includes mitochondrial membrane
potential, we confirmed the results of Castedo et al (J Immunol
1996;157:512-521) that loss of MMP and annexin V positivity are very
closely linked. Again, high ionized calcium was only seen in cells with
depolarized mitochondria. The data are consistent with the proposal that
the mitochondrial permeability transition is a key early event during
programmed cell death, and may indicate the point where cells become
irreversibly committed to death. I guess we should get around to
publishing this some time.
My own feeling is that it is time the flow cytometry community stopped
getting hung up over the concept that measurement apoptosis is an end
in itself. Apoptosis is a dynamic cellular process that is regulated by
genes, and increasingly can be manipulated pharmacologically. The long
term medical significance of this is enormous.
David Hedley
Princess Margaret Hospital/Ontario Cancer Institute
Toronto
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web