 |
 |
 |
 |
 |
Results for ara-C induced apoptosis in leukemic blasts shows that loss of
mitochondrial membrane potential and phosphatidylserine exposure occur
simultaneously, and precede an increase in ionized calcium. This is
slightly different from the results of Castedo et al (J immunol
1996;157:512-521), where annexin V positivity occurred about an hour
after the mitochondrial membrane changes.
You should check the Castedo paper, since they did dual labelling of
annexin V and mitochondrial membrane potential off an argon laser, using
the new dye CMXRos (available from Molecular Probes). Martin Poot
(who will no doubt also respond to this message) has a recent paper in J
Histochem Cytochem that give more detail about CMXRos.
Hope this helps
David Hedley
Ontario Cancer Institute/Princess Margaret Hospital
Toronto
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web