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I have a very basic question, and am hoping it is not too off topic for
this group. I am being asked to analyze by flow, mouse lymphocytes. They
will first require a density gradient procedure to isolate the
lymphoctes.
I will have very little volume of blood to use, and would like to have
an idea as to the minimum volume of whole blood I would need in order to
analyze for CD4, CD8 and CD19 (Ts and total Bs). I sense I can do this
in 2 tubes (experimental and control), so I will need enough for both
tubes.
Does any of you help me?
Please e-mail me privately at echeagaray@sri.org
Thanks in advance!
P. Echeagaray
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web