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I've been trying to isolate CD8+ activated T cells expressing DR
MHCII molecules on their surface using immunomagnetic beads. Unfortunately,
the beads don't readily separate from the cells afterwards in culture
overnight, and I often can't get enough cells for FACS analysis. Does
anyone know of a gentle way of coaxing lymphocytes off from immunomagnetic
beads - one that won't also alter the cells drastically, as I assume
trypsin would? I would greatly appreciate any comments; please send them
directly to :
mvp@nih.gov. Thaks for your time.
Mark Pavlick
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web