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>SYTOX Green resolves cells in the G1, S, and G2 phase of the cell cycle. It
>does so better than PI. We have not yet found a way of distinguishing late
>G2 phase cells from early prophase. Their DNA content is similar; only in
>metaphase the nucleus breaks up and individual chromosomes will be obtained
>(as sub-G1 particles). SYTOX Green works best with the methanol based
>fixation procedure I have outlined.
At the risk of appearing argumentative,..........
I would like to see data, published or otherwise, showing the improved
resolution described. The above statement implies SYTOX Green can resolve
late S phase from G2 and only has problems distinguishing late G2 phase
from mitotics, at least early mitotics. At least that it does so better
than PI. Can it resolve late S from late mitotics, telophase? I'm no fan
of PI and would be very interested in SYTOX Green, if it's better.
td
-- ============================================================================== Thomas Delohery | Internet: t-delohery@ski.mskcc.org Manager, Flow Cytometry Core Facility | Phone: (212) 639-8729 Memorial Sloan-Kettering Cancer Center | Fax: (212) 794-4019 1275 York Ave. Box 98 | New York, NY 10021 | ==============================================================================
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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