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We are using Becton Dickenson's Retic-COUNT kit (Thiazole Orange) to stain =
rat whole blood for reticulocytes. Our baseline values for %retics is =
around 3-4%. A couple animals had baseline values as high as 5.8%. I =
haven't used this assay before and was hoping someone in the flow =
community could tell me if these values are too high for rats (they =
certainly would be for humans) and if we can account for it someway. The =
kit mentions NRBC and/or lymphocytes as possible contaminants, but I'm =
hoping there is some simple solution. Thanks in advance for any ideas or =
suggestions.
tstaley@amgen.com
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web