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M.C.Riedy, K.A.Muirhead, C.P.Jensen, and C.C.Stewart: Use of a Photolabeling
Technique to Identify Nonviable Cells in Fixed Homologous or Heterologous Cell
Populations; Cytometry12:133-139(1991)
I have also heard of a couple other possibilities that I will pass on to you,
but I have no experience with them. If you try one or both let me know how
they are!
1. Christiaan N.Levelt and Klaus Eichmann: Streptavidin-Tricolor is a Reliable
Marker for Nonviable Cells Subjected to Permeabilization or Fixation; Cytometry
15:84-86 (1994)
2. T.J.Fetterhoff, S.P.Holland, K.J.Wile: Fluorescent Detection of Non-Viable
Cells in Fixed Cell Preparations: Cytometry Supplement 6, International Society
for Analytical Cytology:1993 Abstracts (No.204B)
The second reference uses 7-AAD in combination with AD. ie. After staining with
7-AAD (which binds to the dead cells), non-fluorescent AD is added to compete
for binding sites and prevent further uptake of 7-AAD.
Good Luck, and let me know how things go.
Cheryl Smith
University of Toronto
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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