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One of our students has been producing high resolution flow
karyotypes of rat chromosomes from various cell lines.
However, she is now having difficulty with preparations from normal
tissue. Spleen and bone marrow have proved difficult to stimulate
and / or block. Dermal fibroblasts have been cultured and blocked
successfully but the chromosomes are proving difficult to release
free of debris. The method is based on Sillar and Young.
Any comments would be appreciated.
Terry.Dr. Terry Hoy
Principal Research Officer
Department of Haematology Telephone 44 1222 743458
University of Wales College of Medicine FAX 44 1222 744655
Heath Park E-mail hoy@cardiff.ac.uk
CARDIFF CF4 4XN http://www.cf.ac.uk/uwcm/hg/hoy/index.html
U.K.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web