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Does anyone have experience using Molecular Probes LacZ gene expression Kits.
At the moment we are considering using ImaGene Green, and CD3/PE and
HLA-DR/Per-CP for gene product detection in T and Dendrtic cells.
Does anyone have any practical exprience regarding which acyl chain length we
should use - we really don't want to permeabilize the cells. Also, which would
you recommend the DetectaGene kit or the ImaGene kit.
We will be accquiring data on a BD FACScan but later want to sort for gene
expression using a FACStar Plus equipped with a 488nm argon ion laser - we also
have secondary HeNe and HeCd lasers.
Any tips, suggestions would be greatly appreciated.
Thank you
Frank Isdell/Davorka Bilicic
The Rockefeller University\
New York
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web