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Alternatively, you could use H33342, or DAPI. These staines enter PFA-fixed
cells quite readily, so work well with Fix/Perm reagents. These are UV
excitable, unfortunately, so you usually need a multi-laser machine.
Mark KuKuruga, Karmanos Cancer Inst
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web