Re: Ethidium monoazide - Reply

PAUL@flowcyt.cyto.purdue.edu
Tue, 30 Jan 1996 08:13:48

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> I wish to be able to identify dead cells in samples of fixed cells. The
> cells are HIV infected so they have to be fixed before running on the
> machine. I seem to remember ethidium azide can do this, has any one any
> experience of this dye? Who sells it? How do you use it?
>
> Simon Monard
> Aaron Diamond
> New York

Reply:

There is a method listed on page 174 of the Handbook of Flow
Cytometry Methods describing the use of ethidium monoazide -
essentially use it at 5ug/l and place under a while lamp for 10
minutes, wash and fix.

Regards
Paul

J.Paul Robinson, Purdue University Cytometry Labs
robinson@flowcyt.cyto.purdue.edu PH:317-494 6449 FAX:317-494 0517
web http://www.cyto.purdue.edu

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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu