autofluorescence
Antony Bakke (bakkea@ohsu.EDU)
Fri, 26 Jan 96 16:42:24 PST
We are having problems with high levels of autofluorescence in
murine spleen cells from transgenic animals. This shows up as a
cigar shape on an FL1 versus FL2 cytogram with NO antibody or any
fluorchrome present. The transgenics contain the alpha and beta
chain genes for human HLA-DR3.
This phenomena only occurs in the transgenic animals, not in liter
mates and not in other normal mouse strains. Any ideas?
Thanks in advance,
Tony Bakke
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