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I have used a dilute lidocaine solution to remove macrophages from
petri dishes in a larger protocol in which macs were removed from spleen
cell preparations involving plastic adherence. Haven't done it since late
70's and would have to look up references if this is the kind of method
you are looking for. We were able to reuse the macs after this treatment
so I assume that implies gentleness.
Steve Kayes
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/\ /\//\/######/ /\/#######\ ! Stephen G. Kayes, Ph.D. !
/\/ /\//\/ /\/ /\/ ! Structural & Cellular Biology !
/\/ /\//\/ /\/ /\/ ! University of South Alabama !
/\/ /\/ \/#######/ /\/########/ ! Mobile AL 36688 !
/\/ /\/ /\/ /\/ /\/ =================================
\/------\/ /\-----/\/ /\/ /\/ Office: (334) 460-6768
/#######/ \/####/\/ \/ \/ FAX: (334) 460-6771
=========================================================================
On Fri, 26 Jan 1996, RICHARD L. DARLEY wrote:
> Anyone have a trick for either removing macrophages from plastic, or
> better still, preventing them from sticking in the first place.
>
> Surely there must be a better way than scraping them off, which is a
> pain for the small-scale cultures we are running - also I suspect
> many of them get damaged in the process.
>
>
> ################################################################
>
> From: Dr Richard Darley
> LRF Preleukaemia Unit
> Department of Haematology
> UWCM
> Cardiff, CF4 4XN
> U.K.
> tel (+44) 12 22 74 45 24
> Fax (+44) 12 22 74 45 23
> e-mail: darley@cardiff.ac.uk
>
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