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The best positive control of apoptosis are HL-60 cells incubated with
0.15 uM camtothecin for 3 - 4 h. Under these conditions all S phase
cells undergo apoptopsis (positive control) whereas G1 and G2 cells
do not (negative control in the same sample). We usually prepare a
batch of such cells which after 15 min fixation in 1% formaldehyde at
0 degrees is then stored in ethanol at -20 degrees C and lasts for
months as a control in our laboratory. Phoenix Flow Systems
commercially provides such a control. The attemts by us to use the
nucleases treated cells failed to give reproducible results.
Zbigniew Darzynkiewicz
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web