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I am looking for a protocol for=20
>the reliable quantification of bulk DNA/RNA from tissue. I've tried a=20
>standard digestion and extraction followed by spectrophotometric=20
>readings at 260nm. I feel that I am loosing a lot during the=20
>extraction. Does anyone have any infomation that may help?!?!
Try looking up Nucleic Acids Research 19(15) 4293 for a simple method of=20
extracting DNA from mammalian tissue published 1991.=20
Mikael
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web