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Some of the suggestions were to gate out the events that acquired
during this blip since they fell below the normal lymphoid population, but
it definitely changed my percentages. As the machine shifted from linear
to log, monocytes and granulocytes would temporarily acquire in the
lymphocyte gate, so my only option was to re-acquire. Since this "blip"
would occur sometimes in as many as 1 in 4 samples, this option was not
very convenient. Anyway, the BD on-line troubleshooting was great, and
once we got the link established it didn't take them more than a day to
figure out what the problem was.
Anyway, I hope this is helpful. Maybe I can spare another lab a
couple of weeks of "down time!"
Carol
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Carol Oxford _| _| _|_|_|_| _|_|_|
Medical Pathology Flow Cytometry Lab _| _| _| _| _|
University of California _| _| _| _| _|
Davis, California 95616 _| _| _| _| _|
(916) 752-7205 _| _| _| _| _|
(916) 752-4548 fax _|_|_|_| _|_|_|_| _|_|_|
cloxford@ucdavis.edu
University of California, Davis
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web