 |
 |
 |
 |
 |
Eric P Miller
Edinburgh Medical Oncology Unit
"I am Pentium of Borg. Division is futile......you will be approximated."
Star Trek - Generations. Two Captains, one density.
On Wed, 20 Dec 1995 hwortis_sup@opal.tufts.edu wrote:
> Is this a problem that other people have solved?
> We have been using flow to analyze apoptosis in lymphocytes. We
> have been using P.I. staining and using either ethanol or hypotonic lysis
> to obtain nuclei and stained nuclear fragments. The problem is whether
> to use FSC/SSC gates. If the answer is yes, which particles do you omit
> from the gate?
> It seems that there is a real problem of analysis. Each
> apoptotic nucleus may form several fragments. Therefore there
> is no defined relationship between the number of fragments and the number of
> apoptotic cells. Do other flowers recognize this as a problem? If so
> how do you solve it?
>
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web