FACSCAN SIDE SCATTER

mdemaria@warren.med.harvard.edu
Fri, 29 Dec 1995 11:29:11 -0500

We also see this dropping of the side scatter signal on our Facscan. It happens
rarely but it is annoying. I agree with Nancy - if it were a physical problem
like a clog or bubble, then all signals should be affected. To me, it looks more
like communication- the side scatter detector "shuts off" for a second. The
other reason I think this, is that it is exactly the same type of pattern every
time- the signal drops straight down. I don't think a clog or bubble would be
that reproducible.

We also recollect the sample rather than gate.

Hope we find the answer!

Happy New Year

MaryAnn DeMaria
NERPRC
Harvard Medical School

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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu

Home Page Table of Contents Sponsors E-Mail Archive Web Sites

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu