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It is my experience that micro airbubbles in the sheet-fluid can cause
problems during sorting, mainly by drifting of the dropformation, thereby
reduse the purity and yield during a run. I therefore, with good success,
routinously de-gass my sheet-fluid to "no boiling" with a vacumpump and a
cold-trap between the pump and sheetfluid (not to destroy the oil-based
vacumpump). This is actually the speed-vac system adapted to fit the fluid
tank. It might be acceptable to use a standard water-jet pump to create the
vacum for de-gassing.
But, of course, if there are leakages in the system (O-rings, tubes, etc.),
this will cause troubles of unstability. These problems should be easy to
evaluate.
Have a merry X-mas and a happy new flowyear !
Kjartan Egeberg
Inst. of Canser Research and Mol. Biol.
University in Trondheim
N-7005 Trondheim
Norway
T.phone: (47) 73 59 86 70
T.fax: (47) 73 59 88 01
E.mail: Kjartan.Egeberg@ifk.unit.no
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web