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The blast cells which appear during acute transformation of CML have
been charaterized as resembling lymphoblasts (ie. CD10+/CD19+). At other
times the bone marrow phenotype can be quite heterogeneous. In my
experience you could expect to see a wide variety of myeloid precursors
and the proportions of each would be variable over time. I usually
advised our hematologists against monitoring CML patients since it was
difficult to make sense of the dysplastic changes evolving. In an
individual undergoing blast transformation in the setting of a
long-standing myelodysplastic disorder I think the flow would be very
useful since therapeutic decisions may depend on it.
As to your questions about culturing cells, I can't offer any advice.
Good luck.
Michael Weaver
6731 Steveston HIghway
Richmond, B.C.,
V7E 2L2
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web