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We have validated the method listed in Transfusion. The reagents are very
stable and linear to very low EVENT frequencys. We do not stain the CRBCs
with FITC prior to using. The separation in FL1 (BD FACScan) between WBC
nuclei and CRBC nuclei is adequite.
Bill Baughman
703-802-7070 x5807
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Name: AML FCM
E-mail: fcm@amlimmun.dgsys.com
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web