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PI is not irreversibly bound to DNA. At low concentrations (probably
somewhere below 5 ug/ml) the floursence intensity will decrease. It's best
to add it 5-10 min prior to flow analysis. Any storage/fixation can be down
without PI. What, specifically, are you trying to accomplish?
Thomas L. Morgan, Ph.D.
Department of Radiation Oncology
Kaiser Permanente Medical Center
Los Angeles, CA 90027
213-667-3560
morgantl@aol.com
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web