Re: 4 color flow

Kai Toellner (TOELLNKM@novell1.bham.ac.uk)
Fri, 1 Dec 1995 10:04:13 BST

> Begin forwarded message:
>
> Date: Wed, 29 Nov 1995 08:52:33 +0800
> X-Sender: nmilkh@leonis.nus.sg
> X-Mailer: Windows Eudora Version 1.4.4
> To: cyto-inbox
> From: nmilkh@leonis.nus.sg (KH Leong)
> Subject: Re: 4 color flow
> Cc: "David L. Haviland, Ph.D." <HAVILAND@kids.wustl.edu>
>
> ...Although we can detect PerCP on the EPICS, we have not been able to do so
> on our FACStarPLUS! We are still waiting to hear from BD...and meanwhile we
> are trying to figure out the sets of filters and dichroic mirrors for the
> FACStar+. Maybe you or someone else out there could help?
>
> ------
>
> PerCP is very photolabile. 50mW of 488nm light is enough to kill the
> fluorochrome in my experience. Those who have reported that it works have
> used either air-cooled argon lasers or N.D. filters to decrease the power
> output of a water-cooled laser. A 670 band pass filter will work just fine
> for detection of PerCP fluorescence.
>
> Dave Coder
> dcoder@u.washington.edu
>

Dyes which work very well with the FACStarPlus are RED613 and RED670
from Gibco.

Kai Toellner
----------------------------------------------------------------------
Dr. Kai-Michael Toellner
Department of Immunology
The Medical School Tel.: (44) 121 414 6970
University of Birmingham FAX.: (44) 121 414 3599
Birmingham B15 2TT, UK E-mail: K.M.Toellner@bham.ac.uk

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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu

Home Page Table of Contents Sponsors E-Mail Archive Web Sites

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu