kappa/lambda/CD19 reagents
Eric Hsi (ehsi@luc.edu)
Tue, 31 Dec 1996 12:05:57 -0600
I have recently discovered this site. I have a question regarding
reagents. We have been testing the Caltag monoclonal kappa/lambda/cd19
antbody cocktail and comparing it to the same BD combination to try and
get a brighter CD19 signal for use in a clinical flow lab. I have
noticed a couple of cases in which the BD reagent shows no evidence of a
light chain restriction but the Caltag reagent does (usually a very low
percentage, dim kappa). Has anyone else seen this? The population
could not be confirmed with polyclonal kappa and lambda from BD or Tago.
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
