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Has anyone out there in flowland used annexin V to look at
apoptosis in red blood cells? I have a researcher who is looking at
apoptosis in canine RBC's. She is using a commercial source of
annexin V (FITC labelled), and A23187 to induce the translocation of
PS to the cell surface. We are getting no staining whatsoever.
Any input would be greatly appreciated.
Thanks in advance!
Kathy Ragheb
Kathy Ragheb
Purdue University Cytometry Laboratories
HANS B050, West Lafayette, IN. 47907
ph: 317-494-0757 fax: 317-494-0517
ragheb@flowcyt.cyto.purdue.edu
http://www.cyto.purdue.edu
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web