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I have just joined the list. I'm particularly interested in knowing
other workers interested in flow cytometry of plant material
(probably an enthusiastic minority!). I am currently using a Partec CA-III
to determine DNA amounts in roses.
This is a particularly difficult species viz. phenolics. Does anyone
have experience with contolling phenolics in plant tissue as we have
found they cause a great deal of fluorescence quenching, at least
with DAPI staining.
I am also interested to know if Hoechst dyes have a different
permeability to intact plant cells than does DAPI.
Thanks
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web