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Thus, the obvious drawback is how to detach these cells from their substrate
without altering surface proteins. We have tried various enzymatic
treatments using dispase, pronase, collagenase (crude prep.), and mixtures of
these. Cell scraping without treatment destroys the cell population. While
dispase and collagenase are both successful in detaching the cells, I
certainly question the maintenance of epitopic integrity following treatment.
We have received positive (and most importantly, reproducible) results for
some, but not all, of the mAbs tested.
Any suggestions?
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web