 |
 |
 |
 |
 |
I have a user who uses FDG (b-gal) and a surface marker to label some cell
spleen cells. Because would not like to stay until late at night, I'm
looking for a method to keep the cells overnight and analyse them the next
day.
Therefore my questions:
1. Is it possible to fix the cells after the FDG reaction (formaldehyd 2%)?
2. If you keep the cells without fixation at 4° ovn can you still analyse
them on the FACS the next day, or is the FDG product not very stable ?
Any experience on this topic would avoid me to work until late at night....
Thank you very much, and have a nice weekend.
Matthias
______________________________________________________________
Matthias Haury _/_/_/ _/_/_/ _/_/_/
Flowcytometry - Immunology _/ _/ _/ _/ _/
Institut Pasteur Paris _/ _/_/_/ _/_/_/
Email: mhaury@pasteur.fr _/ _/ _/
Tel: 33 (01) 40 61 31 29 _/_/_/ _/ _/
Fax: 33 (01) 45 68 86 39 INSTITUT PASTEUR PARIS
______________________________________________________________
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web