Use of Acridine Orange in killing assay.
Surgical Labs Resident (SURGLAB1@is.rvh.mcgill.ca)
Mon, 18 Nov 96 09:30:00 PST
Hello Flowers,
This is my first time using "the list" and I think it's great,
especially since I am a newcomer to the world of flow cytometry. I'm trying
to adapt an old microscopic technique that uses acridine orange in a
neutrophil killing assay, where candida or staph. a. is used as the target.
A.O. is supposed to passively enter the effector cells, and shift from green
to red fluorescence when phagocytosed targets are killed. Can anyone out
there explain this to me in a little more detail and tell me if it would
work with flow cytometry?
Thanks,
Raffi Manoukian
Royal Victoria Hospital
Montreal, Quebec, Canada
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
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