Mouse reticulocytes
Timothy Graubert (graubert@medicine.wustl.edu)
Tue, 12 Nov 1996 10:46:17 -0600 (CST)
Can anyone suggest a method of detecting mouse reticulocytes which
would not interfere with simultaneous analysis of fluorescein in these
cells? We are analyzing transgenic lines which express LacZ in erythroid
cells and would like to distinguish expression in mature rbc vs. retics.
I gather neither Acridine Orange nor thiazole orange would work in this
setting. How about pyronin Y? Any other reagents? References to
protocols?
Thanks very much.
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
