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Those guys out there that have already experience with the FACScalibur 4
Colors (or equivalent setup on another machine), is there a dye that you
can use on the FL4 to exclude dead cells, while still using FITC, PE,
Tricolor for Surface markers ?
I remember to have heard something about TOPRO etc, but which works the best ?
Thanks for details (dye, manufacturer, compensations, staining concentrations).
Merci,
Matthias
______________________________________________________________
Matthias Haury _/_/_/ _/_/_/ _/_/_/
Flowcytometry - Immunology _/ _/ _/ _/ _/
Institut Pasteur Paris _/ _/_/_/ _/_/_/
Email: mhaury@pasteur.fr _/ _/ _/
Tel: 33 (01) 40 61 31 29 _/_/_/ _/ _/
Fax: 33 (01) 45 68 86 39 INSTITUT PASTEUR PARIS
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web